Antihyperglycemic and antioxidant activities of alcoholic extract of Commiphora mukul gum resin in streptozotocin induced diabetic rats

Bellamkonda, Ramesh; Rasineni, Karuna; Singareddy, Sreenivasa Reddy; Kasetti, Ramesh Babu; Pasurla, Ramatholisamma; Chippada, Appa Rao; Desireddy, Saralakumari

doi:10.1016/j.pathophys.2010.10.002

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Volume 18, Issue 4 , Pages 255-261, September 2011

Antihyperglycemic and antioxidant activities of alcoholic extract of Commiphora mukul gum resin in streptozotocin induced diabetic rats

Ramesh Bellamkonda
- Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
Karuna Rasineni
- Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515003, Andhra Pradesh, India
Sreenivasa Reddy Singareddy
- Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515003, Andhra Pradesh, India
Ramesh Babu Kasetti
- Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
Ramatholisamma Pasurla
- Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
Appa Rao Chippada
- Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
Saralakumari Desireddy
- Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515003, Andhra Pradesh, India
- Corresponding author. Tel.: +91 08554 255879; fax: +91 08554 255805.

Received 1 October 2010; accepted 23 October 2010.

Abstract

Background and objectives: The present study investigated the effect of Commiphora mukul ethanol extract gum resin (CMEEt) on streptozotocin (STZ) induced diabetic rats by measuring fasting blood glucose, plasma insulin, plasma lipid profile, atherogenic index, hepatic lipid peroxidation (LPO), protein oxidation (PO) and activities of enzymatic antioxidants. Methods: Wistar albino rats were divided into 4 groups, normal control group, CM-treated control group, diabetic control group and CM-treated diabetic group. For induction of diabetes, STZ was administered at a dose of 55mg/kg body weight, meanwhile CM-treated groups were administered CMEEt at a dose of 200mg/kg body weight for 60 days. Body weight, plasma glucose and insulin levels were determined in different experimental days, after end of the experimental period the plasma lipid profile and antioxidant enzymes were determined in hepatic tissue. Results: Increase in plasma glucose, total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C), hepatic LPO and PO levels with decrease in plasma high density lipoprotein cholesterol (HDL-C), insulin, hepatic reduced glutathione (GSH) content and activities of antioxidant enzymes namely, glutathione peroxidase (GPX), glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) were the salient features observed in diabetic rats. On the other hand, oral administration of CMEEt at a dose of 200mg/kg for 60 days resulted in the prevention of above mentioned abnormalities. Conclusion: The results suggest that CMEEt could be beneficial in the treatment of diabetes, characterized by atherogenous lipoprotein profile, aggravated antioxidant status and impaired glucose metabolism and in their prevention.